Detailed information on Intracytoplasmic Sperm Injection(ICSI)

Intra Cytoplasmic Sperm Injection (ICSI) is a laboratory procedure developed to help infertile couples undergoing In Vitro Fertilization (IVF) due to severe male factor infertility. ICSI involves the insertion of a single sperm directly into the cytoplasm of a mature egg (oocyte) using a special microinjection pipette (glass needle). After sperm injection into the egg, further culture and embryo transfer is as with the IVF cases. For patients with subfertile semen, this procedure is preferable to IVF.
ICSI can facilitate fertilization by sperm that will not bind to or penetrate an egg. It can also be used to treat men with extremely low numbers of sperm. However, ICSI is generally unsuccessful when used to treat fertilization failures that are primarily due to poor egg quality.

Indications for Intra Cytoplasmic Sperm Injection
Oligospermia - very low sperm counts

Asthenozoospermia – poor sperm motility

Teratozoospermia – too many abnormal sperms

Problems with sperm binding to and penetrating the egg

Antisperm antibodies (immune or protective proteins which attach and destroy sperm) of sufficient quality to prevent fertilization

Prior or repeated fertilization failure with standard IVF culture and fertilization methods

Frozen sperm collected prior to cancer treatment that may be limited in number and quality

Azoospermia with obstructive pathology - absence of sperm secondary to blockage or abnormality of the ejaculatory ducts that allow sperm to move from the testes. In this situation, sperms are obtained from the epididymis by a procedure called microsurgical epididymal sperm aspiration (MESA) or from the testes by testicular sperm extraction (TESE).

Thus very few azoospermic men need to resort to a sperm bank now with the availability of ICSI.

ICSI is not a perfect technique. Some eggs will be damaged by the ICSI process. Some eggs have plasma membranes that are difficult to pierce. In other instances, the fertilized egg may fail to divide, or the embryo may arrest at an early stage of development.

Perinatal outcome studies in Europe suggest that although multiple pregnancies are common with ICSI, there is no evidence of increased incidence of congenital malformations or abnormal karyotype. There is no evidence that abnormalities may arise later in life to babies born as a result of ICSI, although there is also no guarantee that all babies will be normal.

The duration of one IVF or ICSI cycle.

One complete IVF or ICSI cycle takes approximately 15 to 16 days. From Day 1 or 2 of menses the stimulation of the ovaries start by muscular or subcutaneous injections of hormones. The mean stimulation period is 12 days, depending on the reaction of the ovaries. The ovum pick up takes place within two days after stopping the stimulation (usually on day 13). Now the real IVF or ICSI follows in the laboratory. When fertilization occurs, embryos are transferred into the uterus (usually on day 15) and drugs supporting the uterus are given. After approximately 13 days a pregnancy test will show whether the IVF treatment has been successful or not.

For further information on any questions relating to IVF treatment,infertility issues,test tube baby clinic, surrogacy treatment,surrogate mother,surrogacy in India or infertility specialist you may contact
Deccan Fertility Clinic & Keyhole Surgery Center
Tel: +91 22 2446 6633 / 2444 9992
Fax: +91 22 2444 4443
testtubebabyclinic@gmail.com
http://www.testtubebabyclinic.com

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The Deccan Fertility Clinic and Keyhole Surgery Center is India's first budget IVF clinic and an ISO 9001-2000 certified center.We are Infertility clinic, Fertility centre & IVF lab,Surrogacy Clinic for Male & Female in Mumbai,India. Our fertility centre provides the best affordable quality infertility,fertility treatments with advanced reproductive technologies like IVF,ART, GIFT, ZIFT, TET, ICSI,donor egg,surrogate and surrogacy services for all nationalities. http://www.testtubebabyclinic.com